Technical Note Performing the EP stray-light test with potassium chloride on UV-visible spectrophotometers
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چکیده
Where T is transmittance, I0 is intensity of incident light, I is intensity of transmitted light and Is is intensity of stray light. Stray light has an increasing influence on spectroscopic measurements at low levels of intensity, that is, high absorbances. The result is that stray light causes a negative bias in instrument response and eventually becomes the limiting factor for absorbance and therefore concentration that can be measured. The effect of various levels of stray light on measured absorbance compared with actual absorbance is shown in Figure 3. This Technical Note examines the influence of sample composition, sample temperature, bandwidth and wavelength accuracy on the result of the stray-light test using potassium chloride as described in the European Pharmacopoeia (EP).2
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